A simple, rapid, and selective RP-HPLC method was developed for the estimation of acyclovir in human plasma. The method\ninvolves a simple protein precipitation technique. Chromatographic separation was carried out on a reverse phase C18 column\nusing mixture of 5mM ammonium acetate (pH 4.0) and acetonitrile (40 : 60, v/v) at a flow rate of 1.0 mL/min with UV detection\nat 290 nm. The retention time of acyclovir was 4.12 minutes. The method was validated and found to be linear in the range of\n25.0ââ?¬â??150.0 ng/mL.Validation studies were achieved by using the fundamental parameters, including accuracy, precision, selectivity,\nsensitivity, linearity and range, stability studies, limit of detection (LOD), and limit of quantitation (LOQ). It shows recovery at\n91.0% which ismore precise and accurate compared to the other method. These results indicated that the bioanalytical method was\nlinear, precise, and accurate. The new bioanalytical method was successfully applied to a pharmacokinetic linearity study in human\nplasma.
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